FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28777632
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCAGA389250.011.79568769
AGACCAG441150.011.47143369
GTATATA111300.010.7583261
GAGACCA570950.010.4827569
GTATTAG60300.010.4723511
GTATTAA71600.010.0244541
CGAGACC612950.09.80385668
GTATAGA76000.09.761921
GTATTAT121650.09.7579091
CCAGAGA315400.09.64674369
TACCCCA82700.09.6365215
GTATAAA137850.09.4122081
GTATAGG45150.09.3242191
GTATAAT148250.09.2872821
TATAATA170550.09.224532
TAATACT102950.09.0815134
TTATACT140950.09.08084
GTATTAC68850.08.8711451
CAGAGAG303050.08.81049869
ACGAGAC733100.08.76173869
Produced by FastQC (version 0.11.2)