FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35083448
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCA254600.013.98260869
ACTAGGC327000.013.79834169
AGGCATG177250.012.63063869
GACTAGG424700.012.53286969
GTATAAG84450.011.4406871
AGACTAG528450.011.33215969
GTATATA136250.010.9659211
TAGGCAT248250.010.96363569
GTATTAA93700.010.7531821
GTATTAG77900.010.3650681
GTATTAT153500.010.0483061
GGCATGA161250.09.90485569
GTATAGA101950.09.8153211
GAGACTA652200.09.77964269
TTATACT191750.09.7156594
TATACTG131350.09.455545
CTATACT82400.09.336664
GTATAAA173900.09.2862431
ATAATAC162300.09.2466813
GTATTAC84800.09.19615651
Produced by FastQC (version 0.11.2)