FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13600513
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGAG166400.013.93099369
CCTGAGC129300.013.15268969
ACTCCTG298700.012.53027669
CTCCTGA226650.012.14544169
GACTCCT328300.011.7577869
GTATTAA30850.011.6323441
CACGAGC36400.011.08793369
AGACTCC392450.011.0752269
GTATAGA34850.010.3962231
GAGACTC467800.010.17618669
GTATAAT63450.010.1694871
GTATATA49050.010.1300731
GTATTAT55000.010.10071851
GTATAAG32900.010.0684911
GTATATG38250.09.9231681
GTATAAA62100.09.8349181
GGCATAG47950.09.78633855
CTATACT29100.09.7215724
TATTATA72450.09.7143862
GCATAGG48100.09.68412156
Produced by FastQC (version 0.11.2)