FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23303355
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACT205450.013.76851469
GGACTCC139150.013.04012869
CGGACTC163250.012.97461569
GACGGAC259400.012.18161369
GTATTAG50800.011.0727641
GCATAGG75200.010.96622850
GTATATA89100.010.5347291
GTATTAT101900.010.396721
GTATAAT113800.010.370891
GTATAAA115700.010.290061
GGCATAG81000.010.26568155
CATAGGC72650.010.2587751
TATACTG79650.010.1791325
GTATAGA67150.010.1240051
GTATTAC53050.010.0176881
TTATACT111950.010.0158974
TAGGCAT93450.09.93220147
AGGCATA95800.09.86830754
TTAGGCA73500.09.57656546
ATAATAC103300.09.5520643
Produced by FastQC (version 0.11.2)