FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_nic1112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_nic1112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30837130
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA126750.012.8499171
ACTAAGG392900.012.28302669
TTATACT175000.012.2230314
GTATAAG86950.012.1835841
GTATTAG63950.011.9789491
CTAAGGC323000.011.5556569
GACTAAG475000.011.52531969
TAAGGCG260600.011.26483469
TATACTG123050.011.243065
GTATTAT140750.011.1304751
TAATACT116350.011.1196264
ATAATAC134800.010.9797743
GTATAGA90900.010.9708481
TATACAG97850.010.6832545
GTATAAA155750.010.5237821
GTATATG86900.010.5228261
CTAATAC70550.010.1227693
TATACTA69200.010.1207275
GTATTAA87000.010.11409951
GTATAAC62050.010.0656791
Produced by FastQC (version 0.11.2)