FastQCFastQC Report
Tue 23 Jan 2018
H3G35BGX5_n01_ju1872.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3G35BGX5_n01_ju1872.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26321745
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG108600.012.95985669
GTATTAG57800.012.9551861
GTATATA107250.012.676831
GTATTAT118300.012.3386421
TATACTG94100.011.84244255
ATAATAC117600.011.7936653
CTAAGGC147850.011.64257469
GTATAAT144450.011.6099531
ACTAAGG164050.011.58631169
TTATACT138700.011.3427954
TAATACT98950.011.2969434
GTATAGA73900.011.0666361
GTATATG76850.011.0459481
GTATTAC62800.010.824761
GTATTAA68750.010.7913931
GACTAAG208500.010.770720569
TACACAG74350.010.5799185
GTATAAG64750.010.5520581
TATAATA163800.010.4894552
GTATAAA134350.010.4793471
Produced by FastQC (version 0.11.2)