FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28747667
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1009930.3513085079217037TruSeq Adapter, Index 13 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT148400.059.2098542
TCGTATG151900.059.1636543
TCTCGTA148600.059.0604141
CGTATGC152350.059.03469544
ATGCCGT155600.057.8458947
AATCTCG152450.057.16255639
TGCCGTC159800.056.19521748
GCCGTCT159450.055.92960449
TATGCCG161850.055.69688846
CGTCTGA169500.054.64438616
ATCTCGT160300.054.64142640
CCGTCTT163700.054.6240350
GATCGGA173800.053.7150841
ACGTCTG174400.053.10907715
ATCGGAA177200.052.92052
CGTCTTC170650.052.4809751
ACACGTC177550.052.18646213
TCGGAAG189700.049.3596883
CACGTCT190650.048.79938514
CGGAAGA194900.047.8663184
Produced by FastQC (version 0.11.2)