FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32083364
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1246860.38863131684071534TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG181100.058.6338943
CTCGTAT180750.058.32716842
CGTATGC182800.058.1273544
ATGCCGT182700.058.04659347
TCTCGTA181100.058.0431841
ACGGCTA186200.057.5452532
CGGCTAC185150.057.25816333
TGCCGTC189200.055.86908748
GCCGTCT190500.055.46954749
CCGTCTT191200.055.2842550
TATGCCG192400.055.28090346
ATCTCGT189950.055.1566640
CATCTCG192450.054.40548339
TCACGGC198300.054.12056430
CGTCTGA204200.053.46727816
GTCACGG201050.053.4664229
CGTCTTC198350.053.30920851
CACGGCT206550.051.94161631
AGTCACG208750.051.543928
ACGTCTG213300.051.44489315
Produced by FastQC (version 0.11.2)