FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31514978
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2151190.682592892814331TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA341350.063.16575641
CTCGTAT340200.063.084942
TCGTATG337300.062.55415743
CGTATGC353150.062.51151744
ATGCCGT343700.062.36436547
TATCTCG360700.061.67093339
TGCCGTC346100.061.4919448
GCCGTCT334700.061.3287749
TATGCCG362250.061.08402646
ATCTCGT360550.060.83418340
CCGTCTT345100.060.79954550
TCGCGTA24350.060.77989241
CGTCTGA428200.060.09099216
CGTCTTC338550.060.06078351
ACGTCTG434100.059.314115
ACACGTC441650.058.53454613
GCTTATC435500.056.9720436
CGCGTAT25650.056.75767542
CACGTCT455100.056.7135714
GTATGCC400150.055.96252445
Produced by FastQC (version 0.11.2)