FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31266427
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7808482.497400806302556TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1032100.064.3808941
CTCGTAT1036250.064.3722242
TCGTATG1051150.064.362443
CGTATGC1054250.064.157844
CGATCAG1054750.064.13821433
GATCTCG1026500.064.1102739
ATGCCGT1053500.064.09663447
ACGATCA1062200.063.9188532
GTCACGA1063250.063.882129
GATCGGA1091300.063.8403131
TGCCGTC1057600.063.78862448
ATCGGAA1102300.063.7807582
GCCGTCT1057350.063.73835449
TATGCCG1060700.063.6801646
CGTCTGA1078500.063.66077416
ATCTCGT1034900.063.6354540
CACGATC1067050.063.626831
CCGTCTT1061700.063.45425450
TCACGAT1071150.063.40329730
TCGGAAG1106800.063.3181043
Produced by FastQC (version 0.11.2)