FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37727645
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5622061.490169874106905TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1154050.064.7133516
AATCTCG959000.064.6195539
ACACGTC1158900.064.6063213
ACGTCTG1160300.064.3734315
TCTCGTA931250.064.0851341
CTCGTAT927050.063.95495242
TCGTATG915150.063.93118743
CGTATGC961800.063.89816344
CACGTCT1170600.063.8775714
GATCGGA1177600.063.7539251
ATCGGAA1191900.063.6890262
TATGCCG965250.063.60069746
ATGCCGT932550.063.4788847
TCGCGTA71450.063.34817541
TCGGAAG1198800.063.1745763
ATCTCGT961650.063.11319740
GCACACG1190800.063.01872611
CGGAAGA1201250.062.9598164
TGCCGTC930250.062.91190348
GCCGTCT887900.062.76481249
Produced by FastQC (version 0.11.2)