FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33712513
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG16387724.8610200016830545TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2156200.065.7483441
CTCGTAT2167150.065.7473442
TCGTATG2182100.065.7290743
CGTATGC2210850.065.68593644
ATGCCGT2203750.065.5447547
TATGCCG2217650.065.5343146
CATCTCG2172100.065.4676539
GCCGTCT2160000.065.4564549
TGCCGTC2193800.065.44820448
ATCTCGT2171500.065.36880540
CCGTCTT2172300.065.3489750
CGTCTGA2339200.065.3284916
CGTCTTC2148600.065.2180851
ACGTCTG2351350.065.0541115
ACACGTC2362200.064.9636413
CACGTCT2368850.064.6958414
GTATGCC2263450.064.46316545
CACACGT2393200.064.1588412
GCACACG2399650.064.07484411
CTTAGGC2352600.063.84005733
Produced by FastQC (version 0.11.2)