FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34640338
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6627061.9131048894499816TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG885400.064.8352943
CTCGTAT872600.064.8014542
TCTCGTA869100.064.7130241
CGTATGC886800.064.683344
GATCGGA915650.064.601461
ATGCCGT889750.064.404447
CGATCTC863900.064.3363938
CGTCTGA909200.064.296316
GATCTCG865450.064.2848439
CACGATC864000.064.2768536
ACGATCT864600.064.2724237
ATCGGAA928550.064.247332
TGCCGTC893650.064.02994548
TATGCCG898750.063.76210846
GCCGTCT896800.063.74752449
TCACGAT884000.063.7438935
ACACGTC920150.063.73735413
ACGTCTG917400.063.7289415
ATCTCGT875900.063.5481240
CCGTCTT900250.063.5027750
Produced by FastQC (version 0.11.2)