FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26957572
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1873230.6948808297720581TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT254050.063.5499742
TCTCGTA254850.063.3235341
TCGTATG259750.063.29794743
CGTATGC262250.062.72200844
TATGCCG265900.061.899646
AATCTCG263450.061.34875539
ATGCCGT268550.061.17453447
ATCTCGT265150.060.8632240
TGCCGTC273350.060.03698748
CGTCTGA289500.059.1866616
GCCGTCT275050.059.13923649
CCGTCTT277500.058.85309250
ACACGTC295800.058.0076213
CGTCTTC282100.057.5276151
ACGTCTG298650.057.46571715
ATCGGAA304850.056.939132
GATCGGA301550.056.851391
CACGTCT307850.055.80388314
GTATGCC297700.055.56618545
TCGGAAG320300.054.1488233
Produced by FastQC (version 0.11.2)