FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36018293
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1263540.35080507563198515TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG188950.062.36982743
CTCGTAT189650.061.90279842
CGTATGC197700.061.82658844
TCTCGTA191050.061.46689641
ATGCCGT199750.060.2262247
TATGCCG205900.059.51546546
ATCTCGT206950.057.4265740
TGCCGTC208300.057.23966648
CATCTCG214100.056.12161339
CCGTCTT207050.056.101550
GCCGTCT206700.055.97983649
CGTCTGA245500.055.0253516
CGTCTTC210750.054.05276551
ACACGTC254000.053.29282413
ACGTCTG258300.052.31200815
GTATGCC249250.049.59358245
CACGTCT275000.049.2983214
TCGCGTA12800.048.5133541
CGCGTAT13050.048.11295742
ATCGGAA284350.047.7939032
Produced by FastQC (version 0.11.2)