FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35249661
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3092870.8774183672291204TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG409750.064.4927143
TCTCGTA409050.064.3658341
CTCGTAT410850.064.0588242
CGTATGC412250.064.0519344
CGCCAAT415600.063.5255333
ATGCCGT416700.063.23652647
GCCGTCT417200.062.73820549
GTCACGC422750.062.69513329
TGCCGTC419800.062.62887248
TATCTCG421050.062.48293739
ACGCCAA424600.062.43768732
TATGCCG423250.062.37858246
CCGTCTT420100.062.3371750
ATCTCGT424450.062.0622940
TCACGCC433100.061.1564430
CGTCTTC428000.061.07440651
AGTCACG437000.060.82456628
CGTCTGA443500.060.43717216
CACGCCA439500.060.2965131
ACGTCTG452200.059.36594815
Produced by FastQC (version 0.11.2)