FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33047029
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1829950.5537411547646235TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC279050.063.9530144
TCGTATG272950.063.85205543
CTCGTAT250700.063.65638442
TCTCGTA251650.063.30669441
ATGCCGT284100.062.4281147
TATGCCG289100.061.7889146
GATCTCG259550.061.38055839
TGCCGTC288150.061.16690448
GATCGGA308650.060.754491
GCCGTCT285800.060.6318549
CGTCTGA309750.060.57340616
ATCTCGT264400.060.09696240
ACACGTC313900.059.9703913
ATCGGAA316550.059.8930662
ACGTCTG315750.059.48782315
CCGTCTT293950.059.31424750
CGTCTTC293400.058.66195751
TCGGAAG327250.057.8388183
CGGAAGA330650.057.2761544
CACGTCT329000.057.17575514
Produced by FastQC (version 0.11.2)