FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29446502
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5486331.8631516911584272TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT708200.065.3963742
CGTATGC725350.065.36391444
TCGTATG726050.065.32387543
TCTCGTA708650.065.1985141
ATGCCGT727500.064.9867947
GATCGGA744000.064.881511
AATCTCG709800.064.80739
TATGCCG732950.064.6058146
CGTCTGA749150.064.5484816
ATCGGAA755800.064.49422
ACACGTC751400.064.39651513
TGCCGTC734300.064.31428548
ACGTCTG752250.064.310115
ATCTCGT716450.064.2864640
CCGTCTT733400.064.1290350
GCCGTCT734800.064.0214749
CGTCTTC740100.063.4746551
TCGGAAG768950.063.336893
CACGTCT765250.063.2535614
CGGAAGA775600.062.7409934
Produced by FastQC (version 0.11.2)