FastQCFastQC Report
Wed 24 Jan 2018
H3FWTBGX5_n01_ba126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWTBGX5_n01_ba126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35918702
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3684201.0257052161851505TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT508000.065.3297142
CGATGTA543350.065.29510534
TCGTATG507700.065.2058843
TCTCGTA511600.064.9305241
CGTATGC523050.064.70447544
TATCTCG521500.064.4721539
TATGCCG526000.064.3677346
ACCGATG552950.064.3226332
ATGCCGT521450.064.22227547
CCGATGT552700.064.22163433
CACCGAT554050.064.19340531
ATCTCGT527800.063.41375740
TGCCGTC527300.063.2858348
CGTCTGA572900.062.7967716
CCGTCTT526350.062.59326650
GCCGTCT522700.062.52935849
GATCGGA576750.062.295851
TCACCGA572600.062.25330430
ACACGTC578950.062.24201213
GTCACCG575650.061.9477829
Produced by FastQC (version 0.11.2)