FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29465851
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6993222.373330402030472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46000.027.92088315
CGTCTGA46600.027.48680316
ACACGTC51050.025.77558913
CACGTCT52750.024.67970314
CACACGT56750.023.74155612
GCACACG60100.022.94233111
AGGGGGG67700.020.570531
GATCGGA81850.019.8071141
ATCGGAA83850.019.3744962
GTCACGA26200.018.03444529
CGGAAGA91350.017.660954
TCGGAAG94700.016.814583
GAACTCC83550.015.54114521
AGAGCAC98050.015.2404948
CTGAACT89400.014.87592419
TCACGAT27500.014.1274930
CTCGTAT39050.013.98119841
AGCACAC106750.013.80161810
CGGGGGG85000.013.3635711
TGAACTC101850.013.09209720
Produced by FastQC (version 0.11.5)