FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30927959
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377760.1221419104959367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23050.018.52532248
TAGTTAC82650.012.5342099
CGTGAAA36800.012.36504233
TGAAACG36900.012.23657535
ATCCGAA70750.011.92310125
AATCCGA72950.011.8031524
GCCTAAT29200.011.6273621
GCCTTAT167300.011.3604681
CCTTATG143250.011.3119272
CGAGACG88900.010.82627460
GTCCGGA53400.010.8152111
AGTTACG44100.010.7932075
CTTAGTT109850.010.768777
ACGTGAA45500.010.69318232
GTGCATT97000.010.5727561
TTACGGC30500.010.55748940
CTTATGT141500.010.5119353
CGCATAA51750.010.482662
TTAGTTA138250.010.4046198
CACTTAG95600.010.3970625
Produced by FastQC (version 0.11.5)