FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25206351
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA1614030.6403267176593708No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA710090.2817107482157969No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG380690.15102939731339932No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG377350.14970433443539685No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT358530.14223796217072435No Hit
GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA337280.13380754715349316No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT301590.11964841717867056No Hit
GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA273390.10846076054403908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATG420700.028.7925192
GCCTTAT443350.027.8364981
TTACGGC46050.026.8298940
CTTATGT464000.025.9246033
TATGTGA472100.025.709625
ATTACGG50450.025.18398539
GATAGAT486950.025.15522410
ATGTGAT492050.024.9730836
TAGATGC492000.024.85434512
TGTGATA502600.024.4555977
ATGCCTC509050.024.0019715
GTGATAG512750.023.9851448
CGTACGG21200.023.60950923
TAAGTGC543350.022.51493333
ATCTAAG544450.022.4252430
ATAGATG548750.022.41154311
CTAAGTG547200.022.27353932
TCTAAGT552250.022.01950531
TGATAGA567750.021.858799
AGATGCC566750.021.61329813
Produced by FastQC (version 0.11.5)