FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33367540
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32000.016.07845148
CCGTATC36300.013.11313438
TACCGCG20450.012.83692822
TCCGTAT40150.012.72749237
TTAGTTA80600.011.9847098
TAGTTAC62350.011.9000729
GTTACCT64500.011.8833217
GTCGCAA19050.011.3916481
CGAGACG87100.011.33131960
CCGTTAA24950.011.22337930
GTCACAT198550.011.1942171
GTGCATT102850.011.0943831
CCTAGAT117250.010.98488652
GTCCGGA55650.010.6294651
TTACCTT161350.010.5853278
AGTTACC70250.010.3627656
GATCGGA59300.010.2113061
CGTATGA41600.010.18125827
CACTTAG77450.010.1224415
AGTTACG38900.010.0769035
Produced by FastQC (version 0.11.5)