FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27242513
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28900.019.86208548
TAGTTAC92150.017.9265549
TTAGTTA138350.015.8107838
AGTTACC60450.015.1112356
GTTACCT58850.015.04616457
CACTTAG106100.014.9430265
CTTAGTT115400.014.8911227
ACTTAGT112550.014.7707946
TATGGAT116300.014.17518721
TCACTTA111300.014.1190974
TTATGGA117050.014.11392520
TCGTATG43050.013.33346845
TAATTGG121000.013.2200427
ATGGATA128100.013.03337422
AGTTACA133400.012.776834510
CCTTATG120100.012.7348362
TTTTATG131450.012.59401618
ACACGTC49000.012.35667813
CGTACGG24700.012.04507623
GATCGGA51150.012.0433321
Produced by FastQC (version 0.11.5)