FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29917253
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTTGACATAAGAGGCCTCTGTGAGTGGCTGATGGTCAGCCCTGGG372910.1246471392276557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC29150.022.45329740
GATCGGT129400.021.20593350
ATTACGG32100.020.9351939
GACATAA139350.020.569569
CCCGTAT32050.020.094291
AAGCCGT138000.019.75630466
GTCCGGT45150.019.4580291
ATCGGTC145000.019.18947851
GGATCGG144000.019.17748349
ACCACTA143800.019.10545358
TATGCCG17850.019.02000246
ATAAGAG149300.018.82364812
CCGTATT37500.018.2926732
GATTACG32600.018.2523437
CATAAGA160150.018.00725711
CGGTCAC155650.017.89827753
ACATAAG169400.017.70579510
CACTAGA165250.017.66327360
TACAACG37200.017.31221647
GTTCGCT39550.017.0802291
Produced by FastQC (version 0.11.5)