FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32049614
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATT51650.019.0410942
CCCGTAT41600.018.9305571
GTACGTA17050.017.65376717
GCCGTAT42650.015.0998621
CCTTATG78350.014.4731112
GTCCGTT56950.014.4426981
ATTACGG25500.013.58844939
GTCGTTT58950.013.2995941
GTCCGCT88500.013.2092271
TTACGGC21900.013.10520840
CTTATGT90950.012.9682613
GTCGCTT69300.012.8789691
GCCTTAT119550.012.8525461
GATCGGT89600.012.69561150
GACATAA96850.012.3587229
GGATCGG93500.012.27836349
GTCCGGT46500.012.193721
TTATAGC75050.012.171457
GTGCTAT71350.012.11651
GTCCGCA56700.012.0989361
Produced by FastQC (version 0.11.5)