FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35979750
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACA399110.11092628492415872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT40300.029.2691121
TTACGGC58500.028.83791240
ATTACGG62800.026.80764439
TACAACG134900.022.80611847
CCCGTTT76500.022.2818981
ACAACGA143050.021.58015448
GCGTTTT155650.020.8892232
TGTCGGA83200.020.69805324
CGATTGG152600.020.297952
GTGTCGG86750.020.13333723
CAACGAT154450.020.07796349
GCCCGTT60700.020.0666661
GTCGTTT119250.019.9588381
AATTACG84350.019.87586638
CGGATTA62250.019.51078822
GCGGAAT58500.019.445191
GTCCGGT47150.019.375081
CCTATTA163400.019.34142363
TTATTCG73250.019.2093325
TATATCG65600.019.20722617
Produced by FastQC (version 0.11.5)