FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_bgm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_bgm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37698769
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT50350.019.3254551
CCGTATT53100.017.2028732
CCCGTCT112850.015.756021
CCCGTTT88300.015.0232131
CTCCGGT141750.014.54375651
CCCGAAT95200.014.1181771
TCCGGTG109150.013.9482762
CGTATTT95050.013.8449473
CCCGATT28750.013.5135521
TATCCGT41300.013.47461941
CCTTATG86650.013.3694772
CTCGTAT84950.012.9787111
GTCCGTT62550.012.8142311
CCGGTGT104450.012.7665073
CCCGGAT156850.012.5857451
GTATCCG43450.012.5662440
TACGCGA10050.012.53673757
CCGAACG43100.012.5054970
CCCCGAT67000.012.4855441
ATCGGCG39400.012.34736169
Produced by FastQC (version 0.11.5)