FastQCFastQC Report
Thu 22 Aug 2019
H3FWJBGXC_n01_ba183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_ba183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35242647
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28650.010.99456843
CGTATGC28850.010.67593144
CTCGTAT27050.010.48040542
TCTCGTA27200.010.29415241
ATGCCGT31800.09.46552247
TATGCCG39900.08.68431546
TGCCGTC37050.07.93530648
CCGTCTT40200.07.83590350
GCCGTCT39100.07.787805649
CGGCTAC43100.07.146581633
ACGGCTA47000.06.553669532
ATCTCGT42350.06.44632140
CGTCTTC49000.06.21422751
CATCTCG49700.05.774716439
AGTCACG84150.05.11572270
Produced by FastQC (version 0.11.5)