FastQCFastQC Report
Wed 21 Aug 2019
H3FWJBGXC_n01_ba180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_ba180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31474887
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC560010.17792279921449758TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA109700.057.0805131
CGTATGC87250.056.40153544
TCGTATG80050.056.22658543
CTCGTAT81200.055.30078542
CGTCTGA113050.053.99366416
ATCGGAA117050.053.821562
TCTCGTA81300.053.81358341
ATGCCGT89450.053.5667847
ACGTCTG118150.051.988215
TCGCGTA10300.051.65100541
TATGCCG96700.051.28808246
CGCGTAT10950.051.14068642
ACACGTC122000.050.2889313
TATCTCG92300.050.05515339
TGCCGTC96400.048.79717348
ATCTCGT93550.048.30093840
GCCGTCT92300.046.98306349
CCGTCTT94450.046.7657350
TCGGAAG135000.046.5355833
CACGTCT138050.044.7470214
Produced by FastQC (version 0.11.5)