FastQCFastQC Report
Wed 21 Aug 2019
H3FWJBGXC_n01_ba179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_ba179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28989155
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3887091.340877303943492TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA532750.064.077231
ATCGGAA538150.063.5275462
CTCGTAT477550.063.50555842
CGTATGC508500.063.49611344
TCGTATG504750.063.48818643
TCTCGTA459800.063.45443341
CGATCAG513550.063.1676233
ATGCCGT510400.063.0478247
ACGATCA518150.062.7972832
GATCTCG453900.062.73768639
CGTCTGA527150.062.71551516
TATGCCG514000.062.7082746
CACGATC519700.062.6375731
GTCACGA520300.062.53843329
TGCCGTC514900.062.34024448
CCGTCTT512650.062.22437750
TCACGAT524050.062.1780730
ACACGTC535200.062.13659313
ACGTCTG534400.062.047115
GCCGTCT514500.062.04166849
Produced by FastQC (version 0.11.5)