FastQCFastQC Report
Wed 21 Aug 2019
H3FWJBGXC_n01_ba178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_ba178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25527203
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT29850.012.6636351
TTACGAC33550.012.4140135
CCCCCGT44850.011.94011
CGTATTA15350.011.6283893
TGTTACG35100.011.2675393
TACGACT37650.011.2480736
ACGTACG18250.010.93111953
CTCCGAT67750.010.59067251
CGCTAGT11100.010.4056681
CTCGTTT84700.010.3308391
CTCCGGT85550.010.1872831
CTCCTAT57500.010.1654751
CGCCTAT10050.010.0997581
CGTACGA20550.010.04826554
CGTTTCG21300.010.02429731
CTCCCGT86600.010.0233481
CTCGTAT36250.09.9450791
TACGTAC20300.09.82737352
TCGACCG28100.09.46648737
CCCGTCT72900.09.3623761
Produced by FastQC (version 0.11.5)