FastQCFastQC Report
Wed 21 Aug 2019
H3FWJBGXC_n01_ba177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_ba177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31169470
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT7850.017.8351251
CGTATTA23850.017.7564853
CCCGTAT39450.017.567261
GACGTAT33650.016.6417317
ACGATAC67750.016.37568960
CGTATAG35600.015.92690419
ACGTATA36500.015.72591318
TTAACCG26550.015.6867667
CGATACT73050.015.427134561
ACCGTTA28150.014.91943710
CATACGA75050.014.82948457
TAACCGT27600.014.7095678
CGCCTAT14350.014.6347441
CTCGTTT127450.014.3905461
CTCCGGT83750.014.1259511
TAAGCCG66400.014.02125346
CTCGTAT62650.013.9111841
CTCCCGT56650.013.6545731
CCGTTAA30150.013.46543111
CTCCGAT128850.013.337761
Produced by FastQC (version 0.11.5)