FastQCFastQC Report
Wed 21 Aug 2019
H3FWJBGXC_n01_ba176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FWJBGXC_n01_ba176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37141463
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA28750.020.4517143
CCCGTAT48300.018.6963391
GACGTAT37350.018.64780417
CGTATAG38200.018.3248119
ACGTATA40650.017.47838418
TAACCGT31600.017.0564618
TTAACCG33850.016.6464617
ACCGTTA34700.015.33079710
GACTACG46500.015.28025235
GCTACGA35450.014.90834544
CCGTTAA36200.014.88888611
AGACGTA48200.014.7404916
ACGATAC66400.014.28402760
CTCGTTT144650.014.1069811
CCGTATT51750.014.0673532
CTCGTAT73650.014.0195231
ACTACGT50150.013.95869436
AGCTACG40100.013.44116543
CGACTAG39250.013.37529613
CTCCGCT95850.013.3651221
Produced by FastQC (version 0.11.5)