FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33009908
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4532331.3730210941514893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA522690.15834336769432983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG96250.018.6945535
GTACTCT96750.016.61673
TGTACTC99300.016.3767382
TTGTACT103400.014.9557881
GCATTAG113250.013.56318955
TCTAGTT136750.012.9357627
TACTCTA163850.010.8509264
ACGAGAT244900.010.6416097
CGGGAAC150350.010.6316711
CTATGCG78350.010.1128428
GTAGATA276400.09.934796145
TCTATGC89150.09.5538327
CTAGTTG175050.09.2603738
TATGCGA87950.09.2558659
GAACGTA189050.09.1713494
ATCTATG100700.08.900676
TAGAGGT167600.08.7601649
TAGTTGT205100.08.7177489
GGGATCC149050.08.6792811
GGAACGT174650.08.6111963
Produced by FastQC (version 0.11.5)