FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_neg.9.24.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_neg.9.24.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25791295
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC84450.025.9162399
GAGTCTA51550.020.5255094
AACAGAC113800.020.2512235
CGTGTAT186000.019.566643145
AGACATA133600.018.8771978
ACGTGTT104900.018.729204145
GCTGAAT107150.018.4949841
GTACGTT73050.018.360186145
TTGTACT85700.017.5336781
TCGTGTT227950.016.9835145
TAGCATA76250.016.8227948
GTGTCAT74550.016.337559145
ATCTGGT275350.016.133966
CGGATGT95950.016.1145481
GTTACGA91100.015.9899634
CTCCAAC172100.015.8595341
GTAGCAT80950.015.7565287
TCCAACA158450.014.7400222
TCTAGTT121550.014.7267427
CAGACAT184400.014.5806787
Produced by FastQC (version 0.11.5)