FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_neg.7.18.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_neg.7.18.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232709
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC868912.0528460614703254Illumina Single End PCR Primer 1 (97% over 35bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC584441.380770565611763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204800.4838508860401223No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATA55640.13145245751597853Illumina Single End PCR Primer 1 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51780.1223330023396364No Hit
CCACCAACTACCTAATCTGCCATCGGCCGCTCCAATCGCGCGAGGCCCGA48610.11484370883989427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAG96800.0113.1253055
AGCATTA103650.0106.138554
TAGAGGT112300.096.9305959
CCAGCAT123050.089.355612
CAGCATT125400.089.189983
CATTAGA123350.088.9523546
TTAGAGG129150.084.789248
ATTAGAG137900.079.724547
TCCAGCA153900.071.030531
CGCATGT9350.031.77926
AACTACC37250.028.016056
TTGATCC33550.027.4384043
GCTATAT57700.026.00873145
CTACCTA41400.025.907898
CAACTAC41250.025.475035
GATCCCT22600.024.4097771
TCGTTCC19600.024.4038627
CCGTATA28750.024.20795145
TCGCTTA61200.024.02110150-54
AGGTAAC98450.023.57090810-14
Produced by FastQC (version 0.11.5)