FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_neg.11.17.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_neg.11.17.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27869132
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA136300.038.031307145
GGATATC138250.036.306022
GATATCT162750.031.4868853
AGCATTA154750.030.7680364
ATATCTT151250.029.8030074
GCATTAG173400.028.001865
TATCTTG155550.027.860725
GTAGATA575900.027.49392145
GGTGTAT318050.027.353752145
CGGTGGG205450.023.254679145
AGGATAT329250.023.010263145
TCTTGGC200500.022.3013117
CATTAGA216150.022.0947866
TCTCGGG159950.021.711124145
CGGATAT210350.021.1207141
TAGGTTC110850.020.7900964
GGGTGTT247100.020.273857145
CAGCATT284800.018.0187453
GTCTAGG131450.017.7273621
GACACTC76450.017.647092
Produced by FastQC (version 0.11.5)