FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_neg.11.16.18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_neg.11.16.18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24302773
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC279960.11519673084219649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAG181050.033.264935
AGCATTA185200.032.1283874
GGATATC117600.031.02492
TATCTTG121500.029.2879735
ATATCTT119250.029.172234
CTCTATT125050.029.161665145
ATGTGTT228100.028.414513145
GATATCT131400.028.1895033
CATTAGA212800.027.5863886
TGTGCCG449150.026.471615145
TTGTACT185550.026.1341191
TCTAGTT196150.025.9745227
TAGTTGT185400.025.9559619
TGTGTAT122850.025.55293145
TGTACTC203700.024.6769752
TACTCTA223850.023.4405774
GTACTCT220650.023.3247133
CGGTGGG286250.022.920868145
CACTATA119500.022.196889
GAGCATT178900.021.8757118
Produced by FastQC (version 0.11.5)