FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_9424.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_9424.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27590791
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG80250.017.074297145
TGTACGG84050.013.887185145
GTACTCT139350.010.9235853
CGGGAAC113650.010.6030971
CTAGTTG163400.010.6001148
CTCTAGT171050.010.5074096
ACTCTAG178100.010.417015
TCGTGTT143100.010.132495145
TACTCTA176200.09.8301964
AGAGCGG134400.09.763496145
CGTGTAT97900.09.626906145
GGGGAAT237300.09.4832691
GGGATAG167800.09.3444961
TACGTCC766050.09.16103145
CGGGGAT117100.08.8648861
CCGGGAT91000.08.8547371
CCGGGAA123300.08.7135021
TGTACTC206950.08.4119272
GGGGATA210600.08.3694632
GAACGTA105200.08.2667674
Produced by FastQC (version 0.11.5)