FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_7920.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_7920.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25119105
Sequences flagged as poor quality0
Sequence length151
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG1122260.4467754722948926No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1099000.437515588234533No Hit
CGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGA1070400.4261298322531794No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT874380.3480936124117479No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA790620.3147484753139095No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA746010.2969890846031338No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG645910.25713893866839604No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC634460.25258065524229467No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG628890.25036321954942264No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC623390.24817365109147002No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA622530.24783128220531742No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC616230.2453232310625717No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG560590.22317276033521097No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC548290.21827608905651696No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG542960.21615419816908282No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT476920.18986345253941173No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT461500.18372469879002457No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGAC440010.17516945766976968No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTG433150.1724384686476688No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA429000.17078633972030452No Hit
GCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGA426860.16993439853848297No Hit
AGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTT405690.16150655049214532No Hit
GGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGA382510.152278514700265No Hit
AAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCT365140.14536345940669462No Hit
CACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTG363890.14486583021170538No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC362080.14414526313736098No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC359680.14318981508298165No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT355150.14138640688034068No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA348900.13889826090539453No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT343630.13680025621931993No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAA337590.13439571194913194No Hit
TGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCC333480.13275950715600734No Hit
GCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAG321650.12804994445462925No Hit
GAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGAC320050.12741297908504304No Hit
CCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG318090.12663269650729994No Hit
TCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGC311250.12390966955231884No Hit
GCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCG296830.11816901915892306No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA295960.11782266923921056No Hit
CTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTC293980.11703442459434761No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC290970.11583613349281355No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC287710.11453831655228162No Hit
GAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAG286010.11386154084709627No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT282590.11250002736960572No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA280650.11172770685898244No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG279180.1111424949256751No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCC278830.11100315875107812No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC267880.10664392700297244No Hit
GCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGT261960.10428715513550345No Hit
GGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAG254890.10147256440864434No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATC254090.10115408172385124No Hit
GGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCA251980.10031408364270941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATT83700.048.41812145
GAAACTA145600.037.680144
CATGAAT137450.037.179181
ATGAATG143200.035.5054172
TGTGCCG158550.034.019527145
TGTGTAT76550.032.484074145
AGAAACT173750.032.2476123
CTAACCA165450.032.020228
GGGCATA62900.030.9941464
TGAACGA291700.030.733454
GCTGTAA100500.029.9987815
AACTAAC196700.029.8807226
GATGAAC307850.029.850882
TGAATGG171500.029.7966823
ATGAACG298950.029.7743783
AAGAAAC193800.029.5660252
TCCCTGA149750.029.5260873
GGATGAA320050.028.804331
CTCCCTG153100.028.6141872
AAACTAA207750.028.361285
Produced by FastQC (version 0.11.5)