FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_7651.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_7651.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24206958
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT264380.10921653187484358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAG123850.021.1828045
TAGTTGT89750.020.5100429
ACATAAC152350.020.5023216
GTAGATA307850.020.134901145
GTACTCT102150.018.1658213
TAAGTTA129750.018.1129072
CATTAGA138050.017.9013656
TGTACTC112750.016.5335872
AGTTAGA145250.016.4654334
TTGTACT108500.016.4588991
AACATAA202350.016.3675585
GATTGAA210700.016.2692495
TCGTGTT183500.016.198332145
TGGGATC244300.015.8847682
GGGTGTT203000.015.856577145
CAACATA197150.015.8069124
GATCATG147750.015.5490075
GGGAAAA248350.015.413181145
AGGATAT247000.015.350668145
GTTAGAA179000.015.1017295
Produced by FastQC (version 0.11.5)