FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_7651.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_7651.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25696569
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT171450.018.563656145
GTACTCT147050.018.0409553
TACTCTA164800.017.2826844
TGTACTC158550.016.6977352
TCTAGTT183650.015.5874277
TAGTTGT196050.014.0470199
CTAGTTG187500.013.9918378
GTAGATA273150.013.483385145
TTGTACT203650.012.7620271
GTACGTT95000.012.439429145
GTGTGTA220000.012.193138145
CTCTAGT235050.012.1479856
ACTCTAG253850.011.64808755
TCGTGTT209250.011.052528145
GCATTAG158900.010.946095
GACTCAC107450.010.656695
GGGATAG137850.010.3748181
ACTCACT125000.09.9721016
GAGTGTT195100.09.587357145
ATCTCGG807150.09.260637145
Produced by FastQC (version 0.11.5)