FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_7431.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_7431.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21955927
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA732980.33384151805569406No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC610830.27820733781816637Illumina Single End PCR Primer 1 (96% over 33bp)
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT305360.13907861872559513No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC279410.1272594866980565No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT232390.10584385710519077No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT228760.10419054499498016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT146650.041.2211153
TGTACTC157850.038.735992
TTGTACT154300.038.5313341
TAGTTGT156600.038.317859
TACTCTA169350.036.075294
TAATACG79350.034.340644
GAGCATT175600.031.6130818
AGCATTG182500.030.0604579
GAGTCTA76900.029.427942
ATACGAC91850.029.0356276
TCTAGTT225300.027.8881977
TACGACT109750.026.0168257
CTAACCA162700.024.7656548
AGAGCAT236600.023.7689277
ATAATAC127350.023.677193
GTCTAAC95150.023.4983831
AGTCTAA96750.023.1495883
ACGACTC109400.022.9242173
CGACTCA113850.022.7249374
GAAACTA191900.022.0171494
Produced by FastQC (version 0.11.5)