FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_7379.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_7379.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23847309
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA202900.038.9324465
GTGTCCT270900.029.6682934
GATCCTG122400.028.775455
AACGTAT453700.026.2763086
CCATTGT329900.025.359999145
TGATCCT137200.025.2489764
GTATTCA489300.024.571839
ATTCAGT160550.024.3753416
ACATTCA154050.024.1808414
TTACATT150650.024.1682512
GCTCAGA100300.023.8440635
ACGTATT511850.023.7442047
CGATAAT39350.023.3899354
TACATTC160250.022.8862843
CCGGGAA450950.022.6960181
GAACGTA530950.022.6443585
TTGTACT84000.022.6402341
CTTACAT166100.022.3748051
CGTATTC542450.022.2711288
CTACTCT382650.022.2537129
Produced by FastQC (version 0.11.5)