FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_6215.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_6215.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24752195
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT231900.049.833977145
AGTGTAT284600.027.35945145
GATCCTG118000.023.0313645
ATTCTGG90500.022.8226366
CTGGCTC196700.022.4379149
GTAGATA437000.020.903889145
TCGTGTT153000.019.522875145
TAGTGTT163850.019.026548145
TATAGTT288550.017.437187145
GCCAGTA99500.017.043814
GGGAAAA230200.016.629019145
GACAGGC144900.016.6050475
ATGACGT238700.016.577117
AACGTAT389450.016.3012876
ACAGGCG152200.015.9038166
CGTATTC408250.015.6215868
TAACTTG94350.015.5929414
CCTGGCT163750.015.4458848
TTACGAC123850.015.2726675
TACGACT120450.015.22240456
Produced by FastQC (version 0.11.5)