FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_6214.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_6214.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21353197
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG229280.1073750221102723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA88600.025.8482744
AGTCTAA94300.024.1319315
TCCTCTA117350.024.0237989
TTACTTC113850.023.3620644
TACTTCC117900.022.8052185
GAGTGTC114800.022.039621145
CGTGTAT96050.021.435892145
GTCTAAC111950.020.3920356
TTTTACT145750.019.1591072
TCTAACA110800.019.0992897
CTTTTAC153200.018.9522721
GTTACGA66150.018.4059224
AGTGTTA166350.018.260464145
GCCAGTA81450.018.062724
ACTTCCT157850.017.9976436
TCGTGTT82050.017.7598145
TTACGAC69800.017.754765
GTAGCAT68650.017.5243828
TCAGACG67050.017.2939957
CTTGTTA71100.017.25351
Produced by FastQC (version 0.11.5)