FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30982.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30982.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22928319
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAACAAACATGCTAGTAAACTAGCGCCACTTGCGTTCAAAGATCTGA341970.14914743640822514No Hit
TGGATATCTAGGTTCCCATAACGATGAAGAACGCAGCGAAGTGCGATAAG294520.12845250452071955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCT176300.033.7145083
AGCATTA145250.032.9810754
TATCTAG170300.032.810775
AACATGC184150.032.5862469
TGGATAT179450.030.8636131
ACGTCCG195250.029.556828145
CTAGGTT171600.029.436848
GGATATC201300.029.3292662
TAGGTTC183300.028.4672649
CATTAGA171800.027.208986
ATCTAGG197800.027.0765846
CAAACAT240500.027.060577
ATATCTA209000.026.6314244
GAAACAA254700.026.0119253
TCTAGGT199000.025.8935897
AAACATG249500.025.7939668
AACAAAC257400.025.143145
GCCAGTA141650.024.5586324
ACAAACA264400.023.6551136
AGTAGTC145800.023.6108597
Produced by FastQC (version 0.11.5)