FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30979.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30979.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25540686
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG670640.2625771288993569No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA381590.14940475756994157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT199850.058.224891
CATTCAG252300.046.762745
ACATTCA262550.045.3516044
ATTCAGT266350.044.921726
TTACATT266100.044.320372
TTCAGTA275000.042.9289637
TACATTC299550.039.1767853
TCAGTAG363600.032.4284748
CAGTAGC377750.031.2713019
TGTGCCG296900.030.620762145
AACGTAT575050.029.6789235
CCGGGAA472100.028.4764791
GGTTAGA292550.027.8936524
CCATATT157400.027.4182642
GTATTCA635650.026.963488
TATTCAC646350.026.6516599
ACGTATT658700.026.3169486
GAACGTA686150.025.8453244
CGGGAAC640350.024.8599661
TATTCAG223400.024.8489725
Produced by FastQC (version 0.11.5)