FastQCFastQC Report
Tue 27 Aug 2019
H3FTCBGXC_n02_MT_30978.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FTCBGXC_n02_MT_30978.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24080095
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT584880.2428894072054118No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT388540.1613531840302125No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA345670.14355009812046007No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA307460.1276822205228011No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC296260.12303107608171812No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC270200.11220885964112683Illumina Single End PCR Primer 1 (96% over 33bp)
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG246800.10249129000529277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAT340650.042.969242145
AGCATTA131600.042.5140464
GCATTAG168050.033.939295
TGGGATC212650.030.96882
GATCCCG311200.030.7915423
CATTAGA182700.029.948496
GGGATCC339400.028.316431
GGATCCC341800.027.967092
TCCCGAG346750.027.8808425
ATCCCGA361100.027.1745414
ATTAGTT246700.026.977598145
AGTGTAT228850.026.515776145
CCCGAGG375750.026.0183356
CCGAGGC416250.024.6010957
CAGCATT238450.024.4398373
TACGGAC233650.024.2863645
CCAGCAT231950.024.201962
GTGGGAT287300.023.8000351
GCTACGG254700.022.4252263
AACGCCG250650.021.9489443