Basic Statistics
Measure | Value |
---|---|
Filename | H3FTCBGXC_n02_MT_30978.TR3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24080095 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT | 58488 | 0.2428894072054118 | No Hit |
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT | 38854 | 0.1613531840302125 | No Hit |
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA | 34567 | 0.14355009812046007 | No Hit |
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA | 30746 | 0.1276822205228011 | No Hit |
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC | 29626 | 0.12303107608171812 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC | 27020 | 0.11220885964112683 | Illumina Single End PCR Primer 1 (96% over 33bp) |
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG | 24680 | 0.10249129000529277 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGATTAT | 34065 | 0.0 | 42.969242 | 145 |
AGCATTA | 13160 | 0.0 | 42.514046 | 4 |
GCATTAG | 16805 | 0.0 | 33.93929 | 5 |
TGGGATC | 21265 | 0.0 | 30.9688 | 2 |
GATCCCG | 31120 | 0.0 | 30.791542 | 3 |
CATTAGA | 18270 | 0.0 | 29.94849 | 6 |
GGGATCC | 33940 | 0.0 | 28.31643 | 1 |
GGATCCC | 34180 | 0.0 | 27.96709 | 2 |
TCCCGAG | 34675 | 0.0 | 27.880842 | 5 |
ATCCCGA | 36110 | 0.0 | 27.174541 | 4 |
ATTAGTT | 24670 | 0.0 | 26.977598 | 145 |
AGTGTAT | 22885 | 0.0 | 26.515776 | 145 |
CCCGAGG | 37575 | 0.0 | 26.018335 | 6 |
CCGAGGC | 41625 | 0.0 | 24.601095 | 7 |
CAGCATT | 23845 | 0.0 | 24.439837 | 3 |
TACGGAC | 23365 | 0.0 | 24.286364 | 5 |
CCAGCAT | 23195 | 0.0 | 24.20196 | 2 |
GTGGGAT | 28730 | 0.0 | 23.800035 | 1 |
GCTACGG | 25470 | 0.0 | 22.425226 | 3 |
AACGCCG | 25065 | 0.0 | 21.948944 | 3 |